Introducing Custom Recombinant Expression Services at the Cutting Edge of Biomolecular Engineering
Recombinant proteins have a wide range of research clinical applications.
Proteins constitute the basic functional units of an almost endless range of biological processes, including catalyzing chemical reactions, enabling cell membrane transport, mediating cell-cell interactions, forming the structure of cells tissues, controlling cell activity, orchestrating immune responses. Despite their biological pervasiveness, naturally occurring proteins are difficult to extract for research clinical applications, which require large quantities consistent batches. As a result, researchers turn to recombinant protein expression—a bioengineering technique that uses a genetically engineered host cell to express a protein of interest by using the cell’s own protein manufacturing capabilities. Recombinant proteins antibodies are widely used in research as therapeutics in clinical practice. Scientists use recombinant proteins antibodies in myriad ways, including developing producing vaccines therapeutics, performing structural biochemical studies, producing industrial enzymes, advancing food processing technology. One of the most well-known examples of a clinical drug produced through recombinant protein expression is insulin.
Because different proteins have different expression requirements, it may be challenging for any one laboratory to maintain the wide range of skills resources needed for producing various types of proteins. For example, applications that require recombinant antibodies, antibody fragments, or extracellular domains of transmembrane proteins are best suited to mammalian cell expression systems, which have advantages such as high bioactivity low endotoxicity. Alternatively, bacterial expression systems such as E. coli are affordable, rapid, highly scalable, are ideal for applications that require cytokines, enzymes, or bacterial proteins.
Moreover, recombinant protein antibody expression require meticulous optimization standardization at all stages of the expression workflow, from identifying the appropriate host cell, target gene, expression vector to ensuring that the culture conditions, timing, purification processes produce high antibody or therapeutic protein yield quality. Each of these steps is vulnerable to technical challenges, which often limit the throughput, yield, reliability of expressed antibodies proteins. For example, scientists must choose an appropriate expression vector to exert precise control over recombinant protein antibody expression. The vector must be compatible with the expression host organism have a promoter as well as proper translation initiation termination sequences. Adequate reliable expression is often hampered by technical difficulties related to selection of the expression vector, where variations in the size topology of some vectors can result in poor transfection efficiency low yields of expressed proteins antibodies.
High-throughput scaled recombinant production is a valuable resource for preclinical research development.
Sino Biological provides custom recombinant expression services for antibody protein production that adhere to industry quality standards provide scientists with a cost-effective, reliable, versatile resource for preclinical research development work. Sino Biological’s turnkey CRO services include an end-to-end service workflow that incorporates project discussion, proposal confirmation, contract signing prior to project initiation. Sino Biological’s quality control, rapid turnover time, reliable delivery, tried-and-tested recombinant antibody protein expression workflows eliminate the inherent complexity of recombinant technologies, enabling high- throughput large-scale production.
With a broad choice of specially optimized, highly efficient expression systems, including insect cells, E. coli, conventional mammalian cells (HEK293 CHO for transient stable expression), as well as FucoFree HEK293 or CHO to generate afucosylated proteins antibodies, Sino Biological’s recombinant expression services customize antibody protein production for specific applications. Their skilled CRO team walks customers through the best customized approach using multiple touch points to ensure experimental success. Sino Biological’s advanced recombinant protein antibody expression platforms help researchers overcome the technical logistical limitations of recombinant expression, provide a reliable, effective resource for advancing research projects.